Friday, March 29, 2013

If Life were like a lab manual

Camellia sinensis broth protocol

Camellia sinensis broth is an aromatic solution with vaguely medicinal properties. Commonly consumed by the British, many Asians, as well as hipsters trying to be pretentious, this broth is widely used to calm anxiety and is widely accepted as having magical properties. 

Reagents:

H2O liquid buffer, maintained at pH=7
15% lipid content homogenized, heat treated cream
Solid saccharose(C6H12O6)

Preparation of C. sinensis sample
1) Using a mortar and pestle, grind 5-10g of C. sinensis leaves into a powder. 
2) Transfer sample to a .1mm pore size silk filter
3) Seal filter bag completely. 

Preparation of C. sinensis broth

4) Heat H2O liquid buffer(pH=7) to 95C
5) Submerge C. sinensis sample into the buffer.
6) Incubate at room temperature for 4 minutes.

7) Remove sample from the buffer and discard in appropriate waste receptacle.

8) Reheat solution to 80C
9) Vortex to mix thoroughly.

10) Using a volumetric pipette, add 2.5 mL of 15% lipid content cream into solution

11) Using a magenetic stir bar, mix solution until homogeneity is reached.
12) Add 6.0g of solid saccharose(C6H12O6) into solution
13) Mix until all saccharose has been completely dissolved into the solution. 
14) Maintain solution at 80C. 


No comments:

Post a Comment